TADA2A Knockout cell line (Hep G2)

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location: Home > Products > KO Cell Lines > TADA2A Knockout cell line (Hep G2)
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TADA2A Knockout cell line (Hep G2) Order

TADA2A Knockout cell line (Hep G2)
Catalog#
YKO-HT5597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TADA2A Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
TADA2A Knockout cell line (Hep G2)
Species
Human
Gene Name
TADA2A
Gene ID
6871
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 6 (based on transcript TADA2A-205)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TADA2A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TADA2A gene knockout TADA2A Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TADA2A Knockout cell line (Hep G2)
TADA2A Knockout cell line (Hep G2)
Catalog#
YKO-HT5597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    YKO-XN36531(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • TADA2A Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TADA2A
    Gene ID: 6871
    Size: 1*10^6
    Catalog#: YKO-HT8995
  • TADA2A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TADA2A
    Gene ID: 6871
    Size: 1*10^6
    Catalog#: YKO-XN36532
  • TADA2A Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TADA2A
    Gene ID: 6871
    Size: 1*10^6
    Catalog#: YKO-XN36531

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